The effect of standardized ginseng extract on peripheral blood leukocytes and lymphocyte subsets: a preliminary study in young health adults.

The present study was undertaken in twenty young, healthy Thai males to investigate the effects of prolonged administration of standardized ginseng extract on peripheral blood leukocytes and lymphocyte subsets. The subjects were divided into two equal groups as ginseng and placebo groups. The first group received two capsules daily of standardized ginseng extract 150 mg per capsule for 8 weeks. The second group received placebo and served as control. Circulatory levels of total and differential leukocyte counts and percentage of lymphocyte subsets were determined prior to and at the 4th and 8th week of the experimental period. There were no significant differences in the total and differential leukocyte counts as well as the lymphocyte subpopulations: CD3 (T cells), CD 19 (B cells), CD4 (T-helper cells), CD8 (T-suppression cells), CD4/CD8 ratio, and CD25 (Interleukin-2-receptor cells) between the two subject groups throughout the experimental period. We concluded that oral administration of standardized ginseng extract, 300 mg/day for 8 weeks caused no significant changes in peripheral blood leukocytes and lymphocyte subsets in young, healthy Thai males.

Cytochemical and immunological phenotying of acute nonlymphoblastic leukemia in Thai patients.

Using morphology and cytochemical reaction, we could subclassify-according to FAB classification, 51 of our 56 cases of acute nonlymphoblastic leukemia (ANLL) as M1-M5. Five cases were undifferentiated. Using the immunophenotypic method, we could subclassify 51 of these patients as M1-M4. In addition, 3 cases of undifferentiated leukemia by the prior method were each classified as M1, M3, and myelo-megakaryoblastic leukemia. Correlation of ANLL subtype classification according to each method was not good. However, combination of both methods, using immunophenotypic analysis as a supplement would better subclassify the disease. One of the remaining 2 cases of undifferentiated leukemia was also shown to be myelo-megakaryoblastic leukemia by a positive platelet peroxidase reaction by ultrastructural cytochemistry. Thus, combination of these 3 methods could diagnose and subclassify 55 of the 56 cases (98%) of our ANLL patients.